CRISPR-Cas9 Gene Editing workshop

CRISPR-Cas9 Gene Editing workshop

CRISPR is emerging as a rapid diagnostic tool. Due to its simplicity and reproducibility, CRISPR genome engineering could be used successfully to accelerate both basic and applied research in diseases. iGene aims to explore the potential of CRISPR Cas9 technology to combat cancer treatment. We are intensively involved in training Doctoral and graduate students and have successfully applied CRISPR technology to generate several cellular models to study DNA replication dynamics in cancers.
The course will provide insights into the basics of CRISPR gene – editing and online hands-on training in human cell engineering using the robust CRISPR Cas9 system. We will also introduce the attendees to successful sgRNA design and analysis strategies and the state-of-art methods that could be used to address their own genome engineering needs.
This CRISPR beginner’s course is aimed at graduate students and scientists with a good understanding of cellular and molecular biology and an interest in human genome editing. The course is relevant for researchers venturing into genome engineering for the first time.

1. 1. Define a basic workflow to create CRISPR instruments
   2. Demonstrate the efficient RNA guideline selection and design (gRNA)
   3. Provision of human cell CRISPR tools, transfer
   4. Assess genome edit validation in cells engineered